A three-years postdoctoral position is available from early 2023, in the laboratory Repeat Expansions and Myotonic Dystrophy, at the Myology Research Centre in Paris, France.
« Mechanisms underlying CTG repeat instability that involve the formation of R-loops in myotonic dystrophy type 1 (DM1) »
The CTG repeat involved in DM1 becomes pathogenic and highly unstable in somatic tissues and across generations, once above a size threshold. The formation of unusual DNA structures such as slipped-DNAs during different metabolic processes is critical to generate trinucleotide repeats instability. This instability is strongly dependent on tissue and cell types suggesting that DNA replication, DNA repair, transcription as well as chromatin remodelling are involved in the level of instability. In addition, bidirectional transcription at the DM1 locus is known to produce R-loops that promotes instability. Indeed, R-loops can affect transcription by promoting antisense transcription, can be conflictual with replication progression, or may recruit repair mechanisms that recognize these structures as errors. This project aim to dissect the mechanisms and molecular players involved in the regulation of R-loops that could modulate the instability of CTG repeats in experimental cellular and animal models.
The REDS laboratory, headed by D. Furling and G. Gourdon (Web : recherche-myologie.fr) is a leading laboratory in the field of DM1, combining complementary expertise, from genetics and pathophysiology to the development of new therapeutic tools. Our laboratory is located at the Myology Research Centre, a renowned institution dedicated improvement of patient care. The campus of Sorbonne University and Pitie Salpetriere Hospital offer a dynamic environment with state-of-the art facilities and platforms.
Candidates must hold a PhD in a relevant subject (molecular biology, genetics, DNA repair…) and have experience in DNA repair, microsatellites or R-Loops biology. The successful candidate is expected to be highly motivated, well-organised, have excellent communication skills, a high level of accuracy in all aspects of work and the ability to contribute to the project and research group with insight and enthusiasm. He/she will (1) provide input into decisions on experimental approaches and techniques; (2) keep up to date with current developments in the field; (3) collaborate with other members of the group and liaise with external collaborators; (4) take a lead in the presentation of results and paper writing. Good knowledge of the English language is essential.
Salary will be commensurate with experience following Inserm guidelines.
Applications in English should include a cover letter, a detailed CV and the contact details of at least two academic references. Applications and informal inquiries should be e-mailed to Genevieve Gourdon (email@example.com). Please send your application before the May, 31st, 2023.